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Membrane Localized Thaumatin-Like Protein from Tea (CsTLP) Enhanced Seed Yield and Plant Survival Under Drought Stress in Arabidopsis thaliana
(Elsevier, 2021-05-20) Chalo, M.; Asosii, P.; Anish, K.; Sanjay, K.; Biotechnology Division, Council of Scientific and Industrial Research-Institute of Himalayan Bioresource Technology ; Kenya Agricultural and Livestock Research Organization - Tea Research Institute ; Nagaland University
Thaumatin-like proteins (TLPs) are pathogenesis-related (PR5) proteins, which are induced in response to various biotic and abiotic stresses. The present work was carried out to clone TLP of Camellia sinensis (CsTLP) and to evaluate the response of transgenic lines of Arabidopsis constitutively expressing CsTLP under drought conditions. Data showed that transgenic lines exhibited lower relative electrolyte leakage and higher water retention capacity as compared to the wild-type (WT) plants under drought stress. In addition, results with confocal microscopy showed CsTLP + GFP fusion protein to be localized in the cell membrane which moved to the intercellular spaces under prolonged drought stress. Expression of CsTLP enhanced seed yield and the plant survival in transgenic lines as compared to the WT plants under drought stress. Results suggested the importance of CsTLP in improving drought tolerance in Arabidopsis.