Browsing by Author "Beecroft, R.P."
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Item Use of procyclic trypanosomes for detection of antibodies in sera from vervet monkeys infected with Trypanosoma rhodesiense: an immunodiagnostic test for African sleeping sickness(1986) Pearson, T.W.; Liu, M.; Gardiner, I.C.; Longridge, D.; Beecroft, R.P.; Sayer, P.D.; Gould, S.S.; Waitumbi, J.N.; Njogu, A.R.; Kenya Trypanosomiasis Research Institute; Department of Biochemistry and Microbiology of Victoria, Canada, Kenya Trypanosomiasis Research Institute MugugaUncoated procyclic culture forms of African trypanosomes were used in immunofluorescence and simple agglutination assays to detect antibodies in the sera of vervet monkeys infected with T. b. rhodesiense. Antibodies to procyclic surface antigens were found in sera from animals with active, untreated infections or sera taken soon after treatment with trypanocidal drugs. The antibodies were detectable within 7 days of infection. No specific antibodies were detected in sera prior to infection or long after drug cure. The results indicate that antigens expressed on the surface of pro cyclic culture forms of T. brucei spp. Are useful for the detection of antibodies produced in response to infection with T. b. rhodesiense and may allow the development of a simple immunodiagnostic test for African sleeping sickness. In addition, the use of a form of the trypanosome of a different differentiation state from the infecting organism illustrates the utility of this approach for detection of antibodies to common antigens.Item Use of Procyclic Trypanosomes for Detection of Antibodies in Sera from Vervet Monkeys Infected with Trypanosoma Rhodesiense: an Immunodiagnostic Test for African Sleeping Sickness(veterinary Record, 1986) Pearson, T.W.; Liu, M.; Gardiner, I.C.; Longridge, D.; Beecroft, R.P.; Sayer, P.D.; Gould, S.S.; Waitumbi, J.N.; Njogu, A.R.; Department of Biochemistry and Microbiology, University of Victoria, Canada, Kenya Trypanosomiasis Research Institute, Muguga, KenyaUncoated procyclic culture forms of African trypanosomes were used in immunofluorescence and simple agglutination assays to detect antibodies in the sera of vervet monkeys infected with T. b. rhodesiense. Antibodies to procyclic surface antigens were found in sera from animals with active, untreated infections or sera taken soon after treatment with trypanocidal drugs. The antibodies were detectable within 7 days of infection. No specific antibodies were detected in sera prior to infection or long after drug cure. The results indicate that antigens expressed on the surface of procyclic culture forms of T. brucei spp. Are useful for the detection of antibodies produced in response to infection with T. b. rhodesiense and may allow the development of a simple immunodiagnostic test for African sleeping sickness. In addition, the use of a form of the trypanosome of a different differentiation state from the infecting organism illustrates the utility of this approach for detection of antibodies to common antigens.
