Browsing by Author "Brown, C. G. D."
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Item Selection On The Basis Of Major Histocompatibility Type of Lymphoid Cells from a Bovine Chimaera Transformed By Theileria Parasites(1980) Teale,A. J.; Kemp, S. J.; Brown, C. G. D.; Spooner, R. L.In an investigation of the expression of major histocompatibility complex (M.H.C.) antigens by cells infected and transformed in vitro by Theileria parasites, Spooner and Brown (1980) reported that the qualitative expression of such antigens remained unaltered by infection and transformation. However, in one instance cells taken from a dizygous twin and transformed by Theileria parva (Muguga) were found to have a different pattern of reactivity with the Edinburgh panel of Bovine Lymphocyte Antigen (BoLA) typing sera from those of the fresh peripheral blood lymphocytes (PBL) from the donor. Conversely PBL from the same animal transformed by T. annulata (Hissar) exhibited the same BoLA phenotype as uninfected PBL. The donor was a chimaera and it was suggested that the T. parva (Muguga) cell line arose from cells of a minor population within the chimaera, the inference being that this minor population was too small to be detectable in the standard BoLA-typing test. The results of BoLA typing of the parents of the cell donor were consistent with this hypothesis. In an investigation of the minor population hypothesis, manipulation of cell culture populations was attempted by suppression of certain BoLA types within such cultures. The cell donor was male and its twin female, but karyotyping of the PBL of the twins, subsequent to the production of the original cell lines, showed them to comprise 80% female and 20% male cells in both cases. The T. annulata (Hissar) cell line at passage 16, and subsequent to the original BoLA-typing, was shown to be predominantly female although male cells were present, whereas the T. parva (Muguga) cell line at passage 15 was found to be a male cell line, although the possible presence of some female cells was not excluded.Item Transplantation of bovine lymphosarcoma cells to athymic (nude) mice(1977) Irvin, A.D.; Brown, C. G. D.; Kanhai, G.K.; Stagg, D. A.; UNDP/FAO Tick-borne Diseases Project, East African Veterinary Research OrganizationBovine lymphosarcoma cells, previously established in culture, were inoculated subcutaneously into groups of irradiated and non-irradiated athymic (nude) mice. Tumours developed at the site of inoculation in all of the irradiated mice but in none of the others. Tumour growth was progressive in all cases but there was no evidence of invasion of surrounding tissues nor of metastasis. Tumour cells were passaged directly to further mice and a similar growth pattern was recorded. The use of this system suggests a possible small animal laboratory model for bovine lymphosarcoma
