Browsing by Author "Coggins, L."
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Item Growth and Certain Stability Characteristics of African Swine Fever Virus(1966) Coggins, L.; Plum Island Anim. Dis. Lab., Greenport, Long Island, New YorkGrowths of African swine fever (ASF) virus in buffy coat and pig kidney (PK) cell cultures were similar except that the release of virus was delayed in the latter. In buffy coat culture, new cell-associated virus appeared between 6 and 8 hours and soon afterward in the culture fluid. Peak maximal virus titers of 106 hemadsorption5o (HAd5o) /0.1 ml. were obtained in 36 to 72 hours. The virus was refractive to exposure to trypsin ethylenediaminetetraacetate (EDTA), ultrasonic waves and freezing and thawing. A residual amount of virus survived heating at 56 C. for 1 hour. Particle size of ASF virus ranged between 100 and 300 mIsItem Observations on the Epizootiology of African Swine Fever(1965) Heuschele, W.P.; Stone, S.S.; Coggins, L.; East African Veterinary Research Organisation, Muguga. KenyaAn outbreak on a pig farm complying with the pig-paddocking ordinance, designed to prevent contact with the wild pig reservoir, may have been caused by farm labourers killing wild pigs for food. The role of ectoparasites needs further investigation.Item Synthesis and Cytopathogenesis of African Swine Fever Virus in Porcine Cell Cultures(1968) Moulton, J.; Coggins, L.; School of. Veterinary. Medical., University. California,Porcine buffy coat (Be) cells infected with African swine fever (ASF) virus had chromosomal, nucleolar, and cytoplasmic degeneration and developed intracytoplasmic inclusion bodies beginning 12 hours after infection. Cytolysis began 20 hours after infection and was widespread 40 hours after infection. The inclusions stained specifically for deoxyribonucleic acid (DNA) with the Feulgen and acridine orange techniques, resisted deoxyribonuclease digestion, localized fluorescent antibody, and incorporated 3H-Iabeled thymidine. Similar cytologic and cytochemical changes were observed in porcine kidney (PK) 2a cells infected with cell culture-attenuated strains of the virus, but the time sequence of changes was extended. Viral synthesis in Be cells was partially inhibited with 5-iododeoxyuridine (IUDR), 5-fluorodeoxyuridine (FUDR), 5- bromodeoxyuridine (BUDR), and hydroxyurea. Thlaed dition of BUDR to Be cultures at intervals between 2 and 20 hours and ation after 24 hours of infection indicated that viral DNA was synthesize tween 6 and 7 hours, and infectious virus, between 10 and 11 hours.
