Browsing by Author "Crawford, J.G."
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Item Autoradiographic Evidence for the Occurrence of Cell Fusion in Cultures of Theileria-Infected Bovine Lymphoid Cells(1974) Irvin, A.D.; Brown, C.G.D.; Boarer, C.D.H.; Crawford, J.G.; Kanhai, G.K.; East African Veterinary Research Organization, Muguga, Kabete, KenyaTheileria parva-infected bovine lymphoid cells, grown in tissue culture, were labelled with tritiated thymidine. Aliquots of this culture were added to equal volumes of unlabelled cultures of T. parva and T. lawrencei. Smears were prepared from the pure and mixed cultures and examined by autoradiography. Virtually all the cells in the pure labelled cultures had taken tip the tritiated thymidine, and in many cells theilerial macroschizonts were also labelled. Some of the multinucleate cells in mixed cultures appeared to be the product of cell fusion since they contained both labelled and unlabelled nuclei.Item Preliminary Studies on an Atypical Strain of Bovine Theileria Isolated in Kenya(1974) Burridge, M.J.; Brown, C.G.D.; Crawford, J.G.; Kirimi, I.M.; Morzaria, S.P.; Payne, R.C.; East African Veterinary Research Organization, PO Box 32, Kikuyu, Kenya FAO/UNDP Project KEN 22, Veterinary Research Laboratory, PO Kabete, KenyaA theilerial parasite infective for cattle, Theileria sp. (Githunguri), was isolated from the Kiambu District of Kenya through Rhipicephalus appendiculatus ticks. Eight cattle were experimentally infected with Theileria sp. (Githunguri); their reactions were characterized by the production of low numbers of macroschizonts, no microschizonts, few or no piroplasms, and a transient febrile response. All these cattle recovered from clinically mild reactions to infection, but were fully susceptible to subsequent Theileria parva (Muguga) challenge. A cell culture isolate of Theileria sp. (Githunguri) was made from injected lymphoid cells of a reacting animal and, from the resultant in vitro culture suspension, a schizont antigen of the parasite was prepared for the indirect fluorescent antibody test. Serological studies on Theileria sp. (Githunguri), using this test, indicated that it was antigenically distinct from T. parva, T. lawrencei and T. mutans, but had some antigens in common with T. parva and T. lawrencei.
