Browsing by Author "Godfrey, D.G."
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Item Enzyme electrophoresis in characterizing thecausative organism of Gambian trypanosomiasis(1976) Godfrey, D.G.; Veronica, K.; Leister Institute of Preventive Medicine LondonTrypanosoma (Trypanozoon) brucei includes three morphologically identical subspecies which are poorly defined by clinical behaviour; T. b. brucei does not infect man, whereas T. b. rhodesiense causes an acute, and T. b. gambiense a chronic, disease. Thirty-three isolates of the complex, each of which had previously been identified on clinical or other criteria, were compared by the electrophoretic patterns of two trypanosomal enzymes, alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT). One particular ALAT pattern clearly segregated a group of human pathogens of which all except one were labelled T. b. gambiense. The exception was labelled T. b. rhodesiense, and in addition three putative T. b. gambiense isolates did not have this pattern; it is suggested that only one presents a serious anomaly. The T. b. gambiense group could also be subdivided by three ASAT patterns which coincided with known groupings based on serological criteriaItem The Local Reaction in Man at the Site of Infection with Trypanosoma Rhodesiense(1957) Fairbairn, H.; Godfrey, D.G.; West Africa Institute of Trypanosomiasis ResearchA problem of great importance in trypanosomiasis is what happens to the metacyclic trypanosomes when they are injected into a mammal by the bite of an infected tsetse-fly. Gordon and Willett (1956) infected guinea-pigs by the bites of tsetse-flies cyclically infected with Trypanosoma rhodesiense. By the subinoculation of heart blood into rats they showed that the blood of the guinea-pigs was infective 5 minutes, 45 minutes, 4! hours and 24 hours after the infective bite. In a fresh preparation made from the site of the bite within five minutes of its infliction, a single trypanosome was seen; and ground-up tissue, removed from the bitten area within 45 minutes of the infective feed, successfully infected white rats on two occasions. The only occasion on which they found trypanosomes on sectioning the site of bite was immediately after a fly had probed but not fed. They postulated that in guinea-pigs the majority of the metacyclic forms of T. rhodesiense deposited by the feeding tsetse rapidly migrated or were carried away from the site of the bite; that a proportion of these trypanosomes or their descendants reached the general circulation within a few hours, or sometimes within a few minutes, of the infective bite; and that, once the trypanosomes reached the circulation, they persisted in the blood throughout the incubation-period. Their guinea-pigs, however, still had an incubation-period of 7-10 days before trypanosomes were found microscopically in the blood.Item Phospholipids of Trypanosoma lewisi, T. vivax T. congolense, and T. brucei(1967) Godfrey, D.G.The mean lipid content of various isolates of Trypanosoma lell'isi, T. vivax, T. congolellse, and T. brucei ranged from 12.3 to 19.4% of the dry weight. The total lipid phosphorus in most isolations was similar, the means ranging from 2.17 to 2.61 mg of phosphorus per 100 mg of lipid; one isolation of T. vivax, however, had a mean of 2.07, which was significantly different from another of the same species. Five main phosphoruscontaining fractions were separated by thin-layer chromatography; four were identified as the phospholipids: lysolecithin, sphingomyelin, lecithin, and phosphatidyl ethanolamine, while the other fraction could not be identified. Two further minor phosphorus-containing fractions were sometimes seen but their identities were unknown.
