Browsing by Author "McNamara, J.J."
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Item A high prevalence of mixed trypanosome Infections In Tsetse flies in Sinfra, Cote d'Ivoire, detected by DNA amplification(1996) Masiga, D.K.; McNamara, J.J.; Laveissiere, C.; Truc, P.; Gibson, C.W.; Kenya Trypanosomiasis Research Institute; Department of Pathology and Microbiology, School of Veterinary Science, University of Bristol, Longford House, Bristol BS18 7DY, UK, Department of Clinical Veterinary Science, University of Bristol, Longford House, Bristol BS18 7D Y, UK Institut Pierre Richet/OCCGE, BP 1500, Bouaké, Côte d'IvoireThe prevalence of various species and subgroups of trypanosomes in the Sinfra area of Cote d' I voir was determined using the polymerase chain reaction (PCR). Using this technique to amplify specific satellite DNA, targets, it was possible. To identify developmental-stage trypanosomes in the midguts and the proboscides of tsetse without expansion .of parasite populations. The predominant tsetse species in the area was Glossina palpalis, which G pallicera and G. nigrofusca were also present. Microscopical examination of 811 non- teneral flies revealed an infection rate of 14% in midguts and or proboscides. Three subgroups of Trypanosoma congolense (Savannah, Forest & Kilifi), T. simiae, T. gadfreyi, West African T vivax and T brucei ssp. were identified using PCR. T. congolense Forest was the most abundant of the Nannomonas trypanosomes approximately 40 % of all infections were mixed, and there was a significantly higher prevalence of apparently mature T brucei ssp. trypanosomes than has previously been reported. The present study demonstrates the PCR facilitates the easy Identification of mature trypanosome infections in tsetse, providing a reliable estimation of trypanosomiasis challenge.Item A repetitive DNA sequence specific for Trypanosoma (Nannomonas) godfreyi(Elsevier, 1996) Masiga, D.K.; McNamara, J.J.; Gibson, C.W.; Kenya Trypanosomiasis Research Institute; Department of Pathology and Microbiology, School of Veterinary Science, University of Bristol, Langford, UK.The satellite DNA sequence of Trypanosoma (Nannomonas) godfreyi, a recently described parasite of Suidae, was determined. The sequence is 373bp in length, and contains two imperfect internal repeats of approximately 170bp. Like other trypanosome satellite DNAs, it has no extensive open reading frames and is probably non-Coding. There is no significant homology with other major repetitive DNAs within subgenus Nannomonas. We have developed after test that is specific for T. godfreyi and used it to identify the parasite in natural tsetse infections from Zimbabwe and Cote d'Ivoire. This test shows no cross reaction with non-target trypanosomes, even within subgenus Nannomonas, and will be invaluable in studies of the prevalence and I distribution of T. godfreyi.
