Browsing by Author "Mirangi, P.K."
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Item Infection of Kenyan sheep with Ovine herpesvirus 2 (OHV-2) virus.(1966) Mirangi, P.K.; Kang'ee; Kenya Agricultural Research Institute , National Agricultural Research Centre Muguga. P.O BOX 32 Kikuyu.Over 90'X. of all breeds of sheep in Kenya were found to harbour Ovine herpesvirus (OHV-2) virus. In the wildebeest infected areas where malignant catarrhal fever (MCF) is an annual occurrence. 89'Yc, of the sheep were polymerasc chain reaction (PCR) test positive for OHV -2 virus while in regions where wildebeest’s arc not found. 92% of all sheep breeds tested positive for this agent. This OHV-2 specific PCR test did not detect sequences in samples drawn from cattle inoculated with 707K and WC II viruses or cattle showing c1inical wildebeest - associated malignant Catarrhal fever (WA-MCF) or eyen in normal cattle. Primers based on Alcelaphine herpesvirus I (AHV-I) which causes WA-MCF did not recognize DNA from these sheep samples,Item Malignant Catarrhal Fever in Cattle Experimentally Inoculated With a Herpesvirus Isolated From a Case of Malignant Catarrhal Fever in Minnesota USA(1991) Mirangi, P.K.; Rossiter, P.B.; National Veterinary Research Centre: Kenya Agricultural Research Institute, Muguga,A malignant catarrhal fever (MCF)-like syndrome was experimentally induced in three steers, which were under immunization trials with a herpesvirus previously isolated from a case ofMCF in a cow in Minnesota USA. The clinical signs observed in the three steers, and the pathological and histological lesions observed in two of these steers which succumbed to the disease syndrome were indistinguishable from those described for MCF. Although seroconversion was readily demonstrated in the three animals, virus was not re-isolated from the blood leucocytes, secretions and tissues obtained from the two animals which succumbed to the syndrome during the course of the disease and after death. However, a herpesvirus which showed cell rounding cytopathic effects (cpe) in bovine thyroid cells (Bth), was re-isolated from the one steer which survived the disease.
