Browsing by Author "Musoke, A.J."
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Item East Coast Fever of Cattle: 60 Co Irradiation of Infective Particles of Theileria Parva(1973) Purnell, R.E.; Brown, C.G.D.; Cunningham, M.P.; Burridge, M.J.; Musoke, A.J.; Dargie, J.D.; East African Veterinary Research Organization, Muguga, P. O. Kabete, Kenya. Faculty of Veterinary Science, University of Nairobi, Nairobi, KenyaInfective particles (IPs) of Theileria parva, the causative organism of East Coast Fever of cattle, were harvested from the tick vector, Rhipicephalus appendiculatus, using an in vitro feeding technic. In a ranging experiment, pairs of cattle were inoculated with aliquots of suspensions of IPs irradiated at doses of 4–137.6 krad. Doses of irradiation in excess of 8 krad appeared to destroy the parasite. In the 2nd and 3rd experiments, groups of 5 cattle were inoculated with aliquots of suspensions containing low and high concentrations of IPs respectively, irradiated at doses of 4–32 krad. In the 2nd experiment, doses of irradiation in excess of 10 krad appeared to destroy the parasite. In the 3rd experiment, at least 1 animal became infected when inoculated with an aliquot of a suspension irradiated at 16 krad. In all experiments, it appeared that increasing doses of irradiation destroyed increasing numbers of IPs. There was no conclusive evidence that IPs which survived irradiation were attenuated, and it appears that vaccination of cattle against ECF is unlikely to be achieved by inoculation of irradiated IPs using the methods described.Item Immunologic and Pathologic Studies of Infections with Rat-Adapted Plasmodium Chabaudi(Antony J .Musoke, 1973) Musoke, A.J.Progress in the adaptation of Plasmodium chabaudi from the mouse to the rat host was indicated by progressive shortening of the prepatent period of infection, increases in rate of parasite growth, and lengthening of the period of patent parasitemia with successive passages in the new host. The data suggested that the parasite recently taken from the mouse host was more antigenic for rats than were the parasites taken from rats at subsequent passages. Another change noted was that P. chabaudi in rats did not produce the antigen responsible for acquired resistance to Babesia rodhaini as had been noted in infections of mice.Item A Pathogenic Theilerial Syndrome Of Cattle In The Narok District Of kenya(1972) Irvin, A.D.; Brown, C.G.D.; Burridge, M.J.; Cunningham, M.P.; Musoke, A.J.; Pierce, M.A.; Radley, D.E.Two theilerial parasites, pathogenic for cattle, were isolated during a field trial conducted at Aitong in the Narok District of Kenya. In a series of laboratory experiments the first of these parasites was successfully transmitted to all of 18 cattle by syringe inoculation of piroplasm infected blood. Two lines were established which were passaged 5 and 3 times respectively_ The animals inoculated all underwent a haemolytic syndrome with anaemia and jaundice and 5 of them died. Macro- and microschizonts were seen in only one of these 18 animals, but piroplasms were present in large numbers. Animals which had recovered from infection and were challenged with Theileria parva all died and there was no evidence that previous infection had conferred any protection on them. This infection was not transmitted by ticks.
