Browsing by Author "Musyimi, D.M."
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Item Bio Control of Water Hyacinth with Cercospora Piaropi and Myrothecium Roridum Corn Oil Formulations in the Greenhouse for Enhanced Water Resources Management and Conservation(East African Journal of Science, Technology and Innovation, 2021-05-25) Mutebi, C.M.; Musyimi, D.M.; Opande, G.T.; Kenya Agricultural and Livestock Research Organization ; Maseno UniversityWater hyacinth hinders extraction and supply of clean water by clogging intake systems. Despite control efforts, it has remained resurgent and difficult to manage under current methods. Efficacious control for the weed is therefore necessary. This study’s goal was determination of effect of corn oil spore formulations of Cercospora piaropi Tharp and Myrothecium roridum Tode Fries on; disease intensity, relative shoot length and relative biomass of water hyacinth. It was conducted in Maseno University and in a greenhouse at Kibos in Kisumu situated at latitude 00 37’ S and longitude 370 20’ E. The conditions were 25 to 300 C and 22 to 270 C temperature averages during the day and night respectively and 60 to 69% relative humidity. Isolations of C. piaropi and M. roridium were made, grown on PDA, harvested and formulated in corn oil at 1x105, 1x106, 1x107, 1x108 and 1x109 spores/ml. Healthy plants were misted with the corn oil formulations and placed in 90 cm diameter and 1.5 cm depth plastic basins filled with 20 liters of water that had been kept in a container for 24 hours for chlorine to be released. The experimental design was completely randomized design with three replications. Disease intensity, relative shoot length and relative biomass were determined biweekly for 6 weeks. Analysis of variance on the means was done using SAS Institute, Inc.1999 computer software and LSD (p≤0.05) used for mean separation. Disease intensity, AUDPS, relative shoot length and relative biomass scores for both pathogens showed a significant (p ≤. 05) increase as the concentration of spores in the formulations increased. Cercospora piaropi and M. roridum in corn oil formulation at 1x108 and 1x109 spores/ml were found effective for lowering water hyacinth biomass and shoot growth respectively and with potential for use in open waters for water hyacinth control.Item Effect of Cercospora Piaropi Tharp and Myrothecium Roridum Tode Fries Formulated as Corn Oil Emulsion on Water Hyacinth Shoot Growth Under Greenhouse Conditions(SciCell Publishing Company, 2021-03-31) Mutebi, C.M.; Opande, G.T.; Musyimi, D.M.; Kenya Agricultural and Livestock Research Organization ; Maseno UniversityA study was done to find out the comparative effect of Cercospora piaropi Tharp and Myrothecium roridum Tode Fries formulated as corn oil emulsion on water hyacinth shoot growth and biomass under greenhouse conditions. The study site was located in Kibos at latitude 0037’S and longitude 37020’E with average temperature of 25 to 300C and 22 to 270C during the day and night respectively, and 60 to 69% relative humidity. Healthy water hyacinth plants were inoculated with the pathogens formulated in corn oil at 1x109, 1x108, 1x107, 1x106 and 1x105spores/ml. The control plants were not inoculated. The experiment was set up in completely randomized design (CRD) with each treatment replicated three times. At weeks 2, 4, and 6 after inoculation, the average shoot length and biomass for the treated basins were separately compared to the average shoot length and biomass of the control plants. Increase in spore density for both pathogens significantly increased relative shoot length and relative biomass. Relative shoot length was 55.07 and 51.93 for C. piaropi and M. roridum respectively at 1x109 spores/ml while relative biomass was 73.53 for C. piaropi and 37.60 at 1x109 spores/ml. Inoculation suppressed shoot elongation and biomass with 1x109 spores/ml being most effective. Cercospora piaropi formulated in corn oil lowered shoot length and biomass of water hyacinth more than M. roridum did.Item Foliar Diseases and the Associated Fungi in Rice Cultivated in Kenya(MDPI, 2022-05-07) Nganga, E.M.; Kyallo, M.; Orwa, P.; Rotich, F.; Gichuhi, E.; Kimani, J.M.; Mwongera, D.; Waweru, B.; Sikuku, P.; Musyimi, D.M.; Mutiga, S.K.; Ziyomo, C.; Murori, R.; Wasilwa, L.; Correll, J.C.; Talbot, N.J.; Maseno University ; -International Livestock Research Institute ; University of Embu ; Kenya Agricultural and Livestock Research Organization ; International Rice Research Institute ; The University of Arkansas System ; University of East AngliaWe conducted a survey to assess the occurrence and severity of rice blast and brown spot diseases on popular cultivars grown in the Busia, Kirinyaga, and Kisumu counties of Kenya in 2019. Working with agricultural extension workers within rice production areas, we interviewed farmers (n = 89) regarding their preferred cultivars and their awareness of blast disease, as this was the major focus of our research. We scored the symptoms of blast and brown spot and assessed the lodging, plant height, and maturity of the crops (days after planting). Furthermore, we collected leaf and neck tissues for the assessment of the prevailing fungal populations. We used specific DNA primers to screen for the prevalence of the causal pathogens of blast, Magnaporthe oryzae, and brown spot, Cochliobolus miyabeanus, on asymptomatic and symptomatic leaf samples. We also conducted fungal isolations and PCR-sequencing to identify the fungal species in these tissues. Busia and Kisumu had a higher diversity of cultivars compared to Kirinyaga. The aromatic Pishori (NIBAM 11) was preferred and widely grown for commercial purposes in Kirinyaga, where 86% of Kenyan rice is produced. NIBAM108 (IR2793-80-1) and BW196 (NIBAM 109) were moderately resistant to blast, while NIBAM110 (ITA310) and Vietnam were susceptible. All the cultivars were susceptible to brown spot except for KEH10005 (Arize Tej Gold), a commercial hybrid cultivar. We also identified diverse pathogenic and non-pathogenic fungi, with a high incidence of Nigrospora oryzae, in the rice fields of Kirinyaga. There was a marginal correlation between disease severity/incidence and the occurrence of causal pathogens. This study provides evidence of the need to strengthen pathogen surveillance through retraining agricultural extension agents and to breed for blast and brown spot resistance in popular rice cultivars in Kenya.Item Promoting Improved Forages for Increased Livestock Productivity in the Arid and Semi-Arid Lands (ASALs) of Kenya: A Case of Kajiado, Narok, and Taita Taveta Counties(International Journal of Veterinary Sciences and Animal Husbandry, 2023) Maweu, A.N.; Korir, B.K.; Kuria, S.G.; Ogillo, B.P.; Kisambo, B.K.; Wambulwa, L.M.; Kubasu, D.O.; Musyimi, D.M.; Manyeki, J.K.; Chege, P.W.; Kenya Agricultural and Livestock Research OrganizationInadequate nutrition is a major constraint that negatively impacts the growth and viability of cattle farming in Kenya's arid and semi-arid lands (ASALs). This is hastened by the adverse effects of climate change that lead to feed scarcity and poor-quality feed. However, these impacts can only be checked with the use of improved technologies hence the need to disseminate adaptation strategies and technologies. In an attempt to resolve the situation, Kenya Agricultural and Livestock Research Organization (KARLO) in partnership with SNV Netherlands Development Organization agencies instituted a research program; Integrated & Climate Smart Innovations for Agro-Pastoralist Economies and Landscapes Kenya’s (ICSIAPL) and established demonstration plots across highlands and lowland regions in Kajiado, Narok and Taita Taveta counties. This paper focused on evaluating forage nutritive value and its impact on livestock productivity through testing changes in milk yield and animal acceptability for selected grasses and legumes that were promoted. All the grasses established in the lowland regions (Brachiaria camello, Nutrifeed, Sugar graze, Foxtail, Panicum maximum var Maasai) and legumes (cowpea, dolichos lablab and crotalaria) had crude protein above the critical level of 70kg/Dm except Sugargraze which had a Crude protein of 48 kg/Dm. The test animals showed high acceptability to Nutrifeed (83%) relative to the sugar graze (50%) and recorded 33% and 25% increase in milk when fed on nutrifeed and Sugargraze respectively. The highland grasses (Brachiaria cobra, Brachiaria cayman, Boma rhodes, Panicum maximum var Siambaza) and legumes (Desmodium, Lucerne, purple vetch, sweet potato vines and Mucuna) were also high in nutritive quality with Panicum maximum var Siambasa having high animal acceptability of 75% relative to Brachiaria cobra with 20%. Desmodium had animal acceptability rate of 80%. and recorded 23% increase in milk yield. The results inform that legume species promotes animal performance and should thus be included while feeding to boost the production for sustainable ruminant productivity under ASALs ecosystems.
