Browsing by Author "Mutebi, C.M."
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Item Bio Control of Water Hyacinth with Cercospora Piaropi and Myrothecium Roridum Corn Oil Formulations in the Greenhouse for Enhanced Water Resources Management and Conservation(East African Journal of Science, Technology and Innovation, 2021-05-25) Mutebi, C.M.; Musyimi, D.M.; Opande, G.T.; Kenya Agricultural and Livestock Research Organization ; Maseno UniversityWater hyacinth hinders extraction and supply of clean water by clogging intake systems. Despite control efforts, it has remained resurgent and difficult to manage under current methods. Efficacious control for the weed is therefore necessary. This study’s goal was determination of effect of corn oil spore formulations of Cercospora piaropi Tharp and Myrothecium roridum Tode Fries on; disease intensity, relative shoot length and relative biomass of water hyacinth. It was conducted in Maseno University and in a greenhouse at Kibos in Kisumu situated at latitude 00 37’ S and longitude 370 20’ E. The conditions were 25 to 300 C and 22 to 270 C temperature averages during the day and night respectively and 60 to 69% relative humidity. Isolations of C. piaropi and M. roridium were made, grown on PDA, harvested and formulated in corn oil at 1x105, 1x106, 1x107, 1x108 and 1x109 spores/ml. Healthy plants were misted with the corn oil formulations and placed in 90 cm diameter and 1.5 cm depth plastic basins filled with 20 liters of water that had been kept in a container for 24 hours for chlorine to be released. The experimental design was completely randomized design with three replications. Disease intensity, relative shoot length and relative biomass were determined biweekly for 6 weeks. Analysis of variance on the means was done using SAS Institute, Inc.1999 computer software and LSD (p≤0.05) used for mean separation. Disease intensity, AUDPS, relative shoot length and relative biomass scores for both pathogens showed a significant (p ≤. 05) increase as the concentration of spores in the formulations increased. Cercospora piaropi and M. roridum in corn oil formulation at 1x108 and 1x109 spores/ml were found effective for lowering water hyacinth biomass and shoot growth respectively and with potential for use in open waters for water hyacinth control.Item Effect of Cercospora Piaropi Tharp and Myrothecium Roridum Tode Fries Formulated as Corn Oil Emulsion on Water Hyacinth Shoot Growth Under Greenhouse Conditions(SciCell Publishing Company, 2021-03-31) Mutebi, C.M.; Opande, G.T.; Musyimi, D.M.; Kenya Agricultural and Livestock Research Organization ; Maseno UniversityA study was done to find out the comparative effect of Cercospora piaropi Tharp and Myrothecium roridum Tode Fries formulated as corn oil emulsion on water hyacinth shoot growth and biomass under greenhouse conditions. The study site was located in Kibos at latitude 0037’S and longitude 37020’E with average temperature of 25 to 300C and 22 to 270C during the day and night respectively, and 60 to 69% relative humidity. Healthy water hyacinth plants were inoculated with the pathogens formulated in corn oil at 1x109, 1x108, 1x107, 1x106 and 1x105spores/ml. The control plants were not inoculated. The experiment was set up in completely randomized design (CRD) with each treatment replicated three times. At weeks 2, 4, and 6 after inoculation, the average shoot length and biomass for the treated basins were separately compared to the average shoot length and biomass of the control plants. Increase in spore density for both pathogens significantly increased relative shoot length and relative biomass. Relative shoot length was 55.07 and 51.93 for C. piaropi and M. roridum respectively at 1x109 spores/ml while relative biomass was 73.53 for C. piaropi and 37.60 at 1x109 spores/ml. Inoculation suppressed shoot elongation and biomass with 1x109 spores/ml being most effective. Cercospora piaropi formulated in corn oil lowered shoot length and biomass of water hyacinth more than M. roridum did.Item Effect of Different Soil Fertilities on Cowpea Mosaic Virus Disease Incidence(International Journal of Horticultural Science and Technology, 2022) Mutebi, C.M.; Moranga, V.; Kenya Agricultural and Livestock Research Organization ; Kenya Accreditation ServiceThe present study was done to determine the effect of different soil fertilities on cowpea mosaic virus disease incidence. The fertilities were; 50 kg/ha Diammonium phosphate (18.46.0), 50 kg/ha Mavuno (10.26.10) that is a mineral fertilizer that has been extensively utilized in western Kenya, 3 tons/ha farmyard manure (FYM), FYM/Mavuno combination with half rate of the latter at 25 kg/ha, FYM/DAP combination with half rate of the latter at 25 kg/ha and unfertilized control, all applied at planting time. Certified cowpea seed of Kenkunde variety was planted using the different fertilizers in a randomized complete block design (RCBD). Percent cowpea mosaic disease incidence (DI) data was collected and analyzed through one-way ANOVA. The results of cowpea mosaic DI shown as a descriptive statistics collected from the six fertilizer treatments indicated that FYM/DAP combination had the smallest mean for DI (M = 12.55), while DAP alone had the highest DI mean (M = 33.97). Associations among groups in the ANOVA table showed that the fertilizer effects were significant (p < .005). Fisher’s LSD post-hoc tests at 5% level of confidence was used to evaluate the nature of the differences in mean DI. It was observed that 92% of the variance in DI was accounted for the type of fertilizer treatment applied. In conclusion, application of DAP combined with FYM at 25 kg/ha and 1.5 tons/ha can be helpful in lowering the cowpea mosaic virus infection in the region where this study was carried out, though these could be used with knowledge of soil reaction.Item Effect of Nitrogen Level Fertilization on the Intensity of Cercospora Leaf Spot of Mulberry(International Journal of Horticultural Science and Technology, 2021) Mutebi, C.M.; Ondede, D.A.; Kenya agricultural and livestock research organization; Jaramogi Oginga Odinga University of Science and TechnologyLeaf spot (Cercospora moricola, Cooke) is a disease that negatively influences the yield of mulberry (Morus alba L.) plants. To determine the effect of nitrogen levels on the incidence and severity of leaf spot an experiment was carried out on mulberry plants. The nitrogen levels included 0, 100, 200 and 300 kg ha-1, which were applied in two splits coinciding with the two rainy seasons. The study design was randomized complete block design (RCBD) with three replications. Determination of disease intensity involved scoring for disease intensity on a 1-5 Manandhar scale and calculation of the disease incidence were performed by expressing the number of infected leaves as a percentage of the total number of leaves. The values were translated to area under disease progress stairs (AUDPS). The means for AUDPS were subjected to analysis of variance (ANOVA) using PRO GLM in SAS and Fisher’s least significant difference (LSD) used to partition the means at p≤0.05. The results showed that as the rate of nitrogen application was increased, there was a corresponding decrease in AUDPS for disease incidence and a decrease in AUDPS for disease severity. From the obtained results it can be concluded that nitrogen at an application rate of 200 kg ha-1 is an effective approach to suppress Cercospora leaf spot of mulberry and can be recommended to the farmers, where this disease is a problem for cultivation of mulberry.Item Importance of Bio Fertilizers as Alternative Soil Fertility Amendments(SciCell publishing company, 2022) Mutebi, C.M.; Moranga, V.; Kenya Agricultural and Livestock Research OrganizationThere is a current interest in agrarian sustainability with soil microorganisms instead of agro-chemicals. Key constraints in the use of bio fertilizers are; inadequate awareness about bio inoculants and lack of promotion network and publicity among the end users. This review discusses current technical information a way of creating awareness in order to promote the use of bio fertilizers. Publon, Google Scholar, Science Direct and Microsoft Academic data bases were used for the review 2021. Research and review articles published from 2019 onwards were considered as current information for the review. The findings of the review are that; both primary and secondary macronutrients can be provided by bio fertilizers. Potential microbes are; free-living nitrogen fixing bacteria and cyanobacteria, symbiotic nitrogen-fixing bacteria and fungi such as mycorrhiza. Other important functions of bacteria are; conferring to plants the ability for salt tolerance, lignin degradation and remediation of heavy metals from the soil. Bio Compost, vermicompost and termite soil with their rich microorganism content can be used as bio fertilizers for soil nutrient increase. In order to reap maximum benefit from bio fertilizers there is need to formulate them in appropriate materials. Apart from addition of nutrients to the soil, bio fertilizers play an important role in plant health, conclusion. This paper has brought to the fore the need to improve rhizosphere management in a sustainable way particularly at this point in time when there are strong indications that it has deteriorated in the face of continued use of chemical fertilizers.
