Browsing by Author "Ogolla, K.O."
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Item Bloodmeal Host Identities Among Sympatric Glossina Austeni and Glossina Pallidipes Tsetse Flies in Shimba Hills National Reserve, Kwale, Kenya(Frontiers, 2023-04-14) Ogolla, K.O.; Onyango, T.; Bwana, B.K.; Otiende, M.Y.; Mang’era, C.M.; Ochieng, B.; Omolo, M.O.; Mugambi, J.M.; Hassanali, A.; Omondi, P.; Mireji, P.O.; Kenya Agricultural and Livestock Research Organization ; Wildlife Research and Training Institute ; Egerton University ; Masinde Muliro University of Science and TechnologyOdor from preferred/non-preferred tsetse fly vertebrate hosts have been exploited in R&D of attractants/repellents of the fly for human and livestock protection. Odors from vertebrate hosts of Glossina austeni and Glossina pallidipes tsetse flies can facilitate formulation of novel attractants effective against G. austeni or improvement of existing attractant blends for G. pallidipes. We compared vertebrate blood meal sources of both fly species at Shimba Hills National Reserve, Kenya, to establish putative preferred host of either species, hence potential source of G. austeni or G. pallidipes specific odors. We trapped sympatric adult flies in 2021 and 2022 using NGU traps/sticky panels baited with 3-propylphenol, octenol, p-cresol, and acetone (POCA), collected their blood meals and characterized the meals using High Resolution Melting (HRM) vertebrate 16S rRNA- PCR (for host identification), and compared host profiles using GLM and Fisher’s exact tests. We collected 168 and 62 sympatric G. pallidipes and G. austeni with bloodmeal, respectively in 2021 and, 230 and 142 respectively in 2022. In 2021, we identified putative hosts of 65.48 and 69.35% of the G. pallidipes and G. austeni respectively and 82.61 and 80.28%, respectively in 2022. In 2021, we detected harnessed bushbuck, buffalo, common warthog and cattle putative host bloodmeals, and additionally bushpig and suni antelope bloodmeals in 2022. Putative vertebrate bloodmeal sources were significantly different by tsetse fly species (χ²(1, N=457) = 43.215, p < 0.001) and sampling year (χ²(1, N=457) = 8.044, p = 0.005). Frequency of common warthog bloodmeals was higher in G. pallidipes (65.79%) than in G. austeni (38.60%), and that of suni antelope and harnessed bushbuck putative bloodmeals higher in G. austeni (21.05-28.07%) than in G. pallidipes (6.84 - 17.37%) in 2022. There was an apparent change in putative feeding preference/host choices in both fly species between 2021 and 2022. Host bloodmeals in G. pallidipes or G. austeni predominantly from putative harnessed bushbuck, suni antelope or common warthog reveal that these vertebrates have potential odors that can be harnessed and formulated into appropriate attractants for respective species and integrated into routine control regiment for G. pallidipes and/or G. austeni.Item Characterization of Indigenous Chicken Production and Related Constraints: Insights from Smallholder Households in Rural Kenya(Elsevier B.V., 2023-07) Anyona, D.N.; Musyoka, M.M.; Ogolla, K.O.; Chemuliti, J.K.; Nyamongo, I.K.; Bukachi, S.A.; University of Nairobi ; Kenya Agricultural & Livestock Research Organization ; The Co-operative University of KenyaIndigenous chickens (IC) contribute significantly to nutrition and socioeconomic wellbeing of rural households. However, despite their potential, production remains low. Attempts to improve IC production among smallholder farmers in Makueni county, Eastern Kenya have achieved little success due to a variety of constraints. This paper explores IC production characteristics and compares the ranks assigned to production and marketing constraints across geographic regions and in male and female-headed households. A descriptive quantitative household survey of 1217 respondents drawn from IC rearing households was conducted and the results integrated with qualitative findings from 22 informants. Results showed an average flock size of 14.9 ± 15.94 IC per household, with female- headed households having relatively fewer chicken than male-headed households. However, relatively more chicken (15.9 ± 18.9) were lost per household during the last disease outbreak compared to the number kept at the time of study. Production system was largely free-range in nature with minimal provision of supplementary feeds. Disease (1.13±0.5), predation (3.16±1.9) and low market prices (3.89±1.9) were three top ranked (Mean Rank±SD) constraints in that order. Lack of capital, high cost of inputs, poor access to extension services and poor access to knowledge ranked significantly higher in female-headed households and in remote areas, while low market price ranked higher in male-headed households. Failure to agree on the selling price was the major constraint to marketing, while rejection of IC due to diseases, inability to agree on selling price and rejection due to size ranked higher in female-headed households compared to male-headed households. Interventions modeled towards improving biosecurity measures to curb diseases, financial empowerment and facilitating access to markets for smallholder farmers should be prioritized.Item Spatial–Temporal Variations in Parasitological Prevalence and Host-Related Risk Factors of Camel Trypanosomiasis and Its Vectors in North Eastern Kenya: A Repeated Cross-Sectional Study(Hindawi, 2023-04-28) Ogolla, K.O.; Chemuliti, J.K.; Wamwiri, F.N.; Auma, J.E.; Kurgat, R.K.; Wanjala, K.B.; Mugunieri, L.G.; Alusi, P.M.; Mdachi, R.E.; Mukiria, P.W.; Okoth, S.O.; Kenya Agricultural and Livestock Research Organization ; East African Science and Technology Commission (EASTECO)/East African CommunityCamel trypanosomiasis (Surra) is endemic in the Horn of Africa. Understanding the spatiotemporal variations in Surra prevalence, vector dynamics, and host‐related risk factors is important in developing effective control strategies. A repeated cross‐sectional study was conducted to determine the Surra parasitological prevalence, livestock reservoirs, vector density/diversity, and host‐related risk factors in Kenya. Random samples of 847, 1079, and 824 camels were screened at the start of the dry season, peak dry season, and during the rainy season, respectively. Blood samples were examined using the dark ground/phase contrast buffy‐coat technique, and Trypanosoma species were identified based on their movement and morphology in wet and stained thin smears. Reservoir status for Trypanosoma evansi was assessed in 406 cattle and 372 goats. A rainy and dry seasons entomological surveys were conducted to determine the Surra vector abundance/diversity and spatiotemporal density changes. Surra prevalence was 7.1%, 3.4%, and 4.1% at the start of the dry season, peak dry season, and rainy season, respectively. Camel co‐infections by Trypanozoon (T. evansi or Trypanosoma brucei brucei) and Trypanosoma vivax were recorded. Spatial variations in Surra prevalence were recorded at the beginning of dry (X7846,N=2=110.9, p ≤ 0.001), peak dry (X71079,N=2=42.2, p ≤ 0.001), and rainy (X7824,N=2=29.1, p ≤ 0.001) seasons. The screened cattle and goats tested negative for Trypanozoon (T. evansi or T. b. brucei), while two cattle tested positive for Trypanosoma congolense. Biting fly catches were composed of a single species from Tabanus, Atylotus, Philoliche, Chrysops, and Stomoxys genera. The total catches for Philoliche, Chrysops, and Stomoxys were higher in the rainy than dry season consistent with the prevalence results. Surra remains an important camel disease in the region with its prevalence varying in space and time. Camel co‐infections by Trypanozoon (T. evansi or T. b. brucei) and T. vivax necessitate proper diagnosis of suspected cases and targeted therapy.Item Variation of Sensitivity of Trypanosoma evansi Isolates from Isiolo and Marsabit Counties of Kenya to Locally Available Trypanocidal Drugs(PLoS One, 2023-02-02) Mdachi, R.E.; Ogolla, K.O.; Auma, J.E.; Wamwiri, F.N.; Kurgat, R.K.; Wanjala, K.B.; Mugunieri, L.G.; Chemuliti, J.K.; Mukiria, P.W.; Okoth, S.O.; Kenya Agriculture and Livestock Research Organization; East African Science and Technology Commission (EASTECO)\East African Community, Kigali, RwandaTrypanocidal resistance is a major cause of treatment failure. This study evaluated the sensitivity of Trypanosoma evansi field isolates collected from Marsabit and Isiolo counties, Kenya. A total of 2,750 camels were screened using parasitological tests for trypanosomes. Of the screened camels, 113 tested positive from which 40 T. evansi isolates were tested using the single dose mice sensitivity test. Five treatment groups each comprising of 6 mice were inoculated intraperitoneally with 1x105 trypanosomes of each isolate and treated 24 hours later with isometamidium chloride at 1 mg/kg, homidium chloride at 1mg/kg, diminazene aceturate at 20 mg/kg and quinapyramine sulphate & chloride at 1 mg/kg. The fifth group was left untreated (positive control). The mice were monitored daily for 60 days. A survey on camel owners’ practices that influence development of resistance to trypanocidal drugs was then conducted. Results indicated presence of drug resistance in all the 7 study sites that had infected camels. Seven of the isolates tested were resistant to diminazene aceturate whereas, 28, 33 and 34 were resistant to isometamidium chloride, quinapyramine sulphate & chloride and homidium chloride, respectively. Seven (17.5%) isolates of the 40 tested were sensitive to all 4 drugs, whereas, 7.5%, 10%,55% and 10% were resistant to 1,2,3 and 4 drugs, respectively. The prevalence of multiple drug resistance was 75%. Survey data indicated that camel management practices influenced the prevalence and degree of drug resistance. In conclusion, the multiple drug resistance observed in the two counties may not be an indication of total trypanocidal drug failure. Judicious treatment of confirmed trypanosomiasis cases with correct dosage would still be effective in controlling the disease since the observed resistance was at the population and not clonal level. However, integrated control of the disease and the vectors using available alternative methods is recommended to reduce drug use.Item Women’s Empowerment and Intra-Household Gender Dynamics and Practices Around Sheep and Goat Production in South East Kenya(PLoS ONE, 2022-08-04) Ogolla, K.O.; Chemuliti, J.K.; Ngutu, M.; Kimani, W.W.; Anyona, D.N.; Nyamongo, I.K.; Bukachi, S.A.; The Kenya Agricultural Livestock Research Organization ; University of Nairobi ; The Co-operative University of KenyaSmall ruminant production facets like decision-making, ownership, labour allocation, access to- and control over assets are gendered. This study investigates intra-household gender dynamics and practices around sheep and goat production among smallholder farmers in South East region of Kenya. A quantitative study was conducted on 358 dual-headed (married) households to generate gender-disaggregated data on ownership, decision-making and labour allocation around small ruminant production. Qualitative data was collected through focused group discussions to bring out the community perspectives. From the findings, the average number of small ruminants owned by the households as reported by men was slightly higher than women. The average number of small ruminants solely owned by men was significantly higher than by women. Men reported a relatively higher number of jointly owned small ruminants compared to women. More women than men reported that they could give as a gift, sell-off and slaughter jointly owned small ruminants without consulting their spouses. Small ruminants were considered the most important livestock asset in supporting a household’s livelihood by relatively more women than men. Men had more decision-making autonomy over jointly owned small ruminants compared to women. Production tasks around small ruminants such as feeding, watering, selling milk and cleaning housing structures were mostly performed by the women. Qualitative data identified men as the de facto owners of small ruminants with a higher power position in making the important production decisions. The study offers three implications on the design of livestock interventions to empower women, the interventions should ensure that; 1) women are not just owners of livestock assets but also share power and decision-making rights in all aspects of production, 2) production labour is shared equitably between men and women and, 3) women access benefits from livestock production even when animals are owned by men.
