Browsing by Author "Purnel, R.E."
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Item The Application of an Indirect Method of Infecting Ticks with Piroplasms for Use in the Isolation of Field Infections(1974) Cunninggham, M.P.; Purnel, R.E.; Ledger, M.A.; Irvin, A.D.; Brown, C.G.D.; Pyne, R.C.; East African Veterinary Research OrganizationNymphal R. appendiculatus ticks became infected with Theileria parva following the intraperitoneal inoculation of fresh or refrigerated infected bovine blood into rabbits on which the ticks were feeding. The method was successfully used in the isolation of field infections and as blood is the only material needed from such cases the method can be readily and conveniently applied in the isolation of different field strains of T. parva and possibly of similar blood-borne protozoal diseases.Item East Coast Fever: The Infectivity for Cattle of Infective Particles of Theileria Parva Harvested in Various Substrates(1973) Cunningham, M.P.; Brown, C.G.D.; Burridge, M.J.; Joyner, L.P.; Purnel, R.E.; East African Veterinary Research Organization, Muguga, P.O. Kabete, KenyaFemale Rhipicephalus appendiculatus ticks infected with Theileria parva were pre-fed on rabbits for 4 days before being removed and restrained on ‘plasticine’. They were then fed for 2-h periods on capillary tubes containing various substrates, the contents of which were subsequently inoculated into East Coast fever-susceptible cattle. Using this technique, precolostral calf serum and Eagle's Minimum Essential Medium, with the addition of Bovine Albumin Powder, were selected as substrates suitable for further laboratory investigation on East Coast fever as they were acceptable to the ticks and supported the viability of the parasites.
