Browsing by Author "Wesonga, H."
Now showing 1 - 5 of 5
- Results Per Page
- Sort Options
Item Draft Genome Sequences of 2 Strains of Staphylococcus aureus Isolated From Mastitis-Infected Camel in Kajiado County, Kenya(American Society for Microbiology, 2023-07-27) Mwangi, K.; Gachogo, R.; Masila, E.; Ogali, I; Langat, N.; Onywera, R.; Malonza, V.; Wesonga, H.; Maichomo, M.; Murungi, E.; Kenya Agricultural and Livestock Research Organization ; University of Cape Town ; Kisii UniversityWe report the draft genome sequences of two Staphylococcus aureus strains isolated from a mastitis-infected camel in Kajiado County, Kenya. The 2,739,512-bp and 3,025,943-bp draft genomes coding for 2,577 and 2,889 protein sequences, respectively, provide invaluable data for the computational design of a camel mastitis subunit vaccine.Item Draft Genome Sequences of Enterococcus faecium, Enterococcus gallinarum, and Lactococcus lactis Strains Isolated from a Mastitis-Infected Camel in Isiolo County, Kenya(American Society for Microbiology (ASM), 2022-12-10) Maichomo, M.; Gachogo, R.; Masila, E.; Ogali, I.; Otieno, L.; Langat, N.; Onywera, R.; Malonza, V.; Inguyesi, C.; Onyambu, F.; Gicheru, N.; Wesonga, H.; Murungi, E.; Kenya Agricultural and Livestock Research Organisation ; University of Cape Town ; Centre for Molecular Biosciences and Genomics ; University of Nairobi ; Meru University of Science and Technology ; nternational Livestock Research Institute ; Kisii UniversityWe report the draft genome sequences and annotation of Enterococcus faecium, Enterococcus gallinarum, and Lactococcus lactis isolates that were recovered from a mastitis-infected camel in Isiolo County, Kenya. Collectively, these data provide an invaluable repository for data mining to support the development of a potential multicomponent mastitis subunit vaccine.Item Effects of Long Acting Oxytetracycline on Contagious Bovine Pleuropneumonia Experimentally Infected Cattle(Tanzania Journal of Science, 2022-12) Otina, B.; Kitala, P.; Bebora, L.; Olum, M.; Kipronoh, A.; Chesang, L.; Stuke, K.; Wesonga, H.; University of Nairobi ; KALRO-Veterinary Science Research Institute ; GALVmed (Global Alliance for Livestock Veterinary Medicines)Contagious bovine pleuropneumonia is an important disease of cattle. Many strategies employed for its eradication and control have had shortcomings. This study was conducted to determine the effects of long acting Oxytetracycline on its course. The study involved 30 indigenous zebu cattle sourced from an area free of the disease, infected by contact transmission and randomly allocated to Oxytetracycline or saline treatment groups. Clinical observations were recorded on the two groups concurrently. Cattle were tested for the disease using complement fixation test. The mean clinical scores of the groups for each observation was compared post treatment on GENSTAT using unpaired t-test for single sample in groups. Full post-mortem was conducted on the cattle and samples collected for Mmm SC isolation. The clinical scores were worse in the control treatment group; there was no fever in the Oxytetracycline-treated group post treatment. Lesions were observed in 93% of the control and 27% of the Oxytetracycline-treated group. In this study, as in others, Oxytetracycline was shown to lower the severity of the clinical signs of the disease. This is important at slaughter houses meat inspection where decision on whether to pass or condemn the animal is based on the clinical signs and post-mortem findings.Item Genetic diversity and evolution of Mycoplasma capricolum subsp. capripneumoniae strains from eastern Africa assessed by 16S rDNA sequence analysis(2001) Heltandar, M.; Wesonga, H. ; Bolske, G.; Petterson, B.; Karl, E. JMycoplasma capricolum subsp. capripneumoniae (M. capripnelllllol1iae), the causal agent of contagious caprine pleuropneumonia (CCPP). is a member of the so-called Mycoplasma mycoides cluster. These mycoplasmas have two rRNA operons in which intraspecific variations have been demonstrated. The sequences of the l6S rRNA genes of both operons from 13 field strains of M. capripneliinoniae from three neighboring African countries (Kenya, Ethiopia, and Tanzania) were determined. Four new and unique polymorphism patterns reflecting the intraspecific variations were found. Two of these patterns included length differences between the rrnA and rrnB operons. The length difference in one of the patterns was caused by a two-nucleotide insert (TG) in the rmB operon and the length difference in the other pattern was due to a three-nucleotide deletion, also in the rrnB operon. Another pattern was characterized by a polymorphic position caused by a mutation that is known to cause streptomycin resistance in other bacterial species. The strain with this pattern was also found to be resistant to streptomycin. Streptomycin resistant clones were selected from four M. capripneulIloniae strains to further investigate the correlation of this mutation to streptomycin resistance. Mutations in the 16S rRNA genes had occurred in two of these strain s. The fourth pattern included a new polymorphism in position 1059. The results show that polymorphisms in M. capripneumoniae strains can be used as epidemiological markers for CCPP in smaller geographical areas and to study the molecular evolution of this species.Item Indigenous Chicken Production Manual(Kenya Agriculture Research Institute, 2006) Ondwasy, H.; Wesonga, H.; Okitoi, L.; Kenya Agricultural Research InstituteIndigenous chicken farming has been described variously as backyard poultry rearing, rural poultry production or scavenging. For our purpose, any flock of chicken that are kept under free-range management and on which no selection of breeds or improvement by crossbreeding has been done is considered as a flock of indigenous chicken. Indigenous chicken lay between 8 and 15 eggs per clutch depending on availability of feed. They are broody and hatch about 80% of the eggs they sit on. They attain 2-3 clutches in a year.
