Pests and Diseases
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Browsing Pests and Diseases by Subject "Baculovirus"
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Item In Vitro Host Range Studies with a New Baculovirus Isolate from the Diamondback Moth Plutella Xylostella (L.) (Plutellidae: Lepidoptera)(Kenya Agriculture Research Institute, 2000) Kariuki, C.W.; McIntosh, A.H.; Goodman, C.L.; Department of Entomology, University of Missouri, Columbia, Agricultural Research Service, USDA, 65203, Columbia, MissourThe in vitro host range of a newly a newly isolated baculovirus from the diamondback moth Plurella xylostella wa tested against six lepidopteran cell lines.Two baculaviruses with wide host ranges from the alfafa looper Autographa califormica(A. californica multiple nuecleopolyhedrovirus, AcMNPV)and the celery looper Anagrapha falcifera (AfMNPV)were also included in this study for comparative purpose, PxMNPV replicated in all six cell lines and produced occlusion bodies.with HV-AM1 AND TN-CL1 cells producing the highest viral titers and greatest number of occlusion bodies. There was no significant replication of AcMNPV and AfMNPV in the HZ-FB33 cell line and thus no production of occlusion bodies. Tje restriction endonuclease profiles of the three baculoviruses showed similarities but could be readily distinguished from each other. Either HV-AM1 or TN-CL1 would be suitable cell lines for the in vitro production of PxMNPV.Item Infectivity Studies of a New Baculovirus Isolate for the Control of the Diamondback Moth (Plutellidae: Lepidoptera)(veterinary Record, 1999) Kariuki, C.W.; McIntosh, A.H.; Department of Entomology University of Missouri, ColombiaThis study describes a new baculovirus isolate recovered from infected larvae of the diamondback moth, Plutella xylostella (L.), and identified as a multiple nucleopolyhedrovirus (MNPV). The plaque purified isolate designated as PxMNPVCL3 was found to be pathogenic to P. xylostella, Heliothis virescens (F.), Trichoplusia ni (Hübner), H. subflexa (Guenée), Helicoverpa zea (Boddie), Spodoptera exigua (Hübner), and S. frugiperda (J. E. Smith) larvae in decreasing order of susceptibility. The LC50 for diamondback moth, the most susceptible, was 6 occlusion bodies (OB)/cm2, whereas the most resistant species, namely S. frugiperda, was 577 OB/cm2. PxMNPVCL3 was more pathogenic to diamondback moth by 3–4 log cycles as compared with 2 broad-spectrum baculoviruses, namely Autographa californica (alfalfa looper) MNPV and Anagrapha falcifera (celery looper) MNPV. The 3 baculoviruses were compared with each other and characterized by restriction endonuclease (REN) analysis, hybridization, and neutralization tests. Fragmentation profiles generated by REN showed that the 3 baculoviruses shared some fragments in common. Hybridization studies employing digoxigenin labeled PxMNPVCL3 DNA as a probe revealed the close but distinct relationship of these 3 viruses. Neutralization tests confirmed the hybridization studies, namely that the 3 viruses although genetically similar are distinguishable from each other.
