Microtitre Techniques for the Assay of Rinderpest Virus and Neutralising Antibody

Abstract

Microtitre techniques were compared with conventional tube techniques for their ability to assay rinderpest virus and neutralising antibody to the virus. The microtitre technique was as sensitive and reliable for assaying the virus as the recommended tube technique, using cell suspensions. Both of these methods, however, were less sensitive than tube titrations on preformed cell monolayers. The microtitre test was as sensitive as the tube test for detecting and assaying virus neutralising antibody and more robust in that it was less sensitive to variations in virus dose.