The Application of Monolayer Tissue Culture Techniques in Rinderpest Research

Abstract

The second technique (II), described by Plowright and Ferris, was used to passage rinderpest virus of the Kabete « 0 » strain and ill the production of high-titre virus stocks. Experiments designed to ascertain some of the factors governing virus yield in culture and losses in freeze drying will be described elsewhere. Titrations were carried out in primary calf-kidney cultures using a technique similar to that described in the above reference. However, minor modifications were introduced during the last 2 years or so, so that each culture tube received 0.2m.l of virus inoculum instead of 0.1 ml and negative tubes were not discarded until after 11 or 12 days of observation, compared with the original 8 days. Normally 5 tubes were used per dilution but the number was increased to 10 when simultaneous titrations were performed in cattle and tissue cultures.