Growth of Rinderpest and Bovine Virus Diarrhea Viruses in Theileria Parva Infected Iympho blastoid Cell Lines

Abstract

CO-Cultivation of lymphoid cells from infected cattle on feeder monolayers of bovine spleen or thymus is a routine technique for establishing continuous lines of lymphoblastoid cells transformed and infected by the protozoan Theileria parva (Malmquist and Brown 1977, Kurtti and others 1981). Recently several viruses, including bovme herpesvlfus-3 (also classified as bovid herpesvirus-4) and rinderpest vaccine virus have been isolated from such cultures following development of cytopathic effects in the monolayers (P. B. ROSSISTER and others, m preparation). It would have been interesting to know whether these viruses originated from the monolayers or the transformed Iympho blasts or other cells in the culture but it was not possible to determine this. A small study was subsequently made to investigate whether two lympho tropic viruses, rinderpest virus and Bovine virus diarrhoea virus (BVDV) could infect and grow in T parvaI~ infected Iympho blastoid cell lines which, once established, grow continuously in suspension without a requirement for feeder layers.