The Antigenicity of Mycoplasma mycoides III-Isolation of Precipitating Antigens from Urine.

Abstract

Precipitation of urine with increasing volumes of iso-propyl alcohol, removing the precipitate at each step and deproteinization with chloroform and butanol, produced a number of fractions which gave specific precipitin hands in the agar gel precipitin test when diffused against hyperimmune sheep sera, and which were Molisch positive and biuret negative. The number of precipitin bands produced with the different fractious depended on the amount of alcohol used for the precipitation. In a series of experiments in which the precipitate produced by each volume of alcohol was removed before the next volume of alcohol was added, 1 and 2 volumes of alcohol produced fractions which gave 6 precipitin bands, while 3 volumes produced a precipitate which gave 4 hands or occasionally 5. Precipitates from 4 and 5volumes produced 4 bands, whereas 6 volumes gave only 2 bands. P.1/2/3, the result of precipitation with 3 volumes of alcohol added in one aliquot, gave 6 precipitin bands, whereas P.4/5/6, the result of precipitation with a further 3 volumes of alcohol followed by alternate precipitation with 3 and 6 volumes, gave 2 strong and 2 weak precipitin bands.