Studies with a strain of Contagions Pustular Dermatitis Virus in Tissue Culture

Abstract

A description is given of the growth and cytopathogenicity of an English strain of contagious pustular dermatitis (C. P. D.) virus in mono• layer cultures derived from lam b and calf testes and the kidney" of embryo-nic sheep, calve:; and goat kids. This strain behaved very similarly to other mammalian pox-like virus: under investigation. The infection was disseminated more rapidly and completely in cultures of testis cells and peak titres were often "lightly higher in this cell type. Some evidence produced suggested that adsorption of viral inocula to testis cells was more complete than to kidney cells. Growth curve studies in sheep kidney and testis cell' are described in detail. The amount of virus-l demonstrable in the fluid was consistently less than that in attached cells throughout the phase of logarithmic' increase and for a considerable time thereafter.