Notes on Immunoprecipitin Reactions with Rinderpest Virus

Abstract

Clear precipitin lines in counterimmunoelectrophoresis could be detected within 25 min if the distance between antigen and antibody wells was reduced to 3 mm. The test was most sensitive between 30° and 50°C. The microslide test was considered too slow for routine use, but was sensitive in detecting and separating different precipitins. Inclusion of 2 mm of phenylmethyl-sulphonylfluoride with the positive control antigens helps to maintain potency, particularly when refrigeration is inadequate.