A Microtitre Technique for the Assay of Malignant Catarrhal Fever Virus and Neutralising Antibody

dc.bibliographicCitation.endpage232en
dc.bibliographicCitation.stpage230en
dc.bibliographicCitation.titleVeterinary Researchen
dc.bibliographicCitation.volume27en
dc.contributor.authorMushi, E.Z.
dc.contributor.authorPlowright, W.
dc.contributor.institutionRoyal Veterinary College, London
dc.date.accessioned2015-07-15T09:29:17Z
dc.date.available2015-07-15T09:29:17Z
dc.date.issued1979en
dc.description.abstractA microtitre technique for the quantal assay of a cell-free strain of malignant catarrhal fever virus was developed, using serially passaged bovine embryonic kidney cells. End-points were determined after 12 days’ incubation and the mean titre recorded for a single virus stock stored at −70°C over a six-month period was 105·5±0·2 (SD). In neutralisation tests serum/virus mixtures were best held at 37°C for 1 h in microtitre trays before the addition of cells; assays were highly reproducible, figures of 101·5±0·2 being obtained for a single reference serum.en
dc.description.notesPathology Department, Royal Veterinary College, Londonen
dc.identifier.citationMushi, E.Z., Plowright, W. (1979). A Microtitre Technique for the Assay of Malignant Catarrhal Fever Virus and Neutralising Antibody. Research in veterinary science, 27(2), 230-232. https://doi.org/10.1016/S0034-5288(18)32835-2en
dc.identifier.doihttps://doi.org/10.1016/S0034-5288(18)32835-2
dc.identifier.issn0928-4249*
dc.identifier.urihttps://kalroerepository.kalro.org/handle/0/4526
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subject.agrovocMalignant catarrhal fever virusen
dc.subject.agrovocAntibodiesen
dc.subject.agrovocNeutralizationen
dc.subject.agrovocBlood serumen
dc.titleA Microtitre Technique for the Assay of Malignant Catarrhal Fever Virus and Neutralising Antibodyen
dc.typeJournal Contribution*
dc.type.refereedRefereeden
dc.type.specifiedArticleen

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