Genome Characterisation of Two Complete Coding Sequences of Tomato Mild Mottle Virus from Tree Tomato and their Distribution in Kenya

Kinoga, M.N.; Kuria, P.K.; Miano, D.W.; Kiambi, R.G.; Mollov, D.S.; Grindstead, S.; Wasilwa, L.A.

Genome Characterisation of Two Complete Coding Sequences of Tomato Mild Mottle Virus from Tree Tomato and their Distribution in Kenya

dc.contributor.author	Kinoga, M.N.
dc.contributor.author	Kuria, P.K.
dc.contributor.author	Miano, D.W.
dc.contributor.author	Kiambi, R.G.
dc.contributor.author	Mollov, D.S.
dc.contributor.author	Grindstead, S.
dc.contributor.author	Wasilwa, L.A.
dc.contributor.institution	Kenya Agricultural and Livestock Research Organization ; University of Nairobi ; U.S. Department of Agriculture ; National Germplasm Resources Laboratory
dc.date.accessioned	2024-07-31T06:43:23Z
dc.date.available	2024-07-31T06:43:23Z
dc.date.issued	2023-02
dc.description	journal article
dc.description.abstract	We present here the first complete coding sequence of tomato mild mottle virus (TMMoV) infecting tree tomato in Kenya. A survey was conducted in three tree tomato-growing regions: Nairobi, Eastern, and Rift Valley. Leaf samples displaying mosaics, mottling, and malformation, were collected and analysed using high throughput RNA sequencing, which revealed the presence of TMMoV in the Eastern and the Rift Valley regions. Two coding sequences, MW537584 and MW537585 were assembled, and the HTS data were verified using RT-PCR and Sanger sequencing. Phylogenetic analysis revealed that both of these Kenyan isolates were highly similar to the Ethiopian isolate (NC038920) with 97 and 96% sequence identity, respectively. Samples from two farms that tested positive for TMMoV, also tested positive for potato virus Y (PVY), making it difficult to associate the symptoms observed with TMMoV, PVY, or a co-infection of both.
dc.description.sponsorship	USAID
dc.identifier.citation	Kinoga, M.N., Kuria, P.K., Miano, D.W., Kiambi, R.G., Mollov, D.S., Grindstead, S. & Wasilwa, L.A. (2023). Genome Characterisation of Two Complete Coding Sequences of Tomato Mild Mottle Virus from Tree Tomato and their Distribution in Kenya. Journal of Plant Pathology, 105(1), 15–19. https://doi.org/10.1007/s42161-022-01265-1
dc.identifier.doi	https://doi.org/10.1007/s42161-022-01265-1
dc.identifier.uri	https://kalroerepository.kalro.org/handle/123456789/14055
dc.language.iso	en
dc.publisher	Springer
dc.subject	Potato virus Y
dc.subject	RNA sequencing
dc.subject	Phylogenetic analysis
dc.title	Genome Characterisation of Two Complete Coding Sequences of Tomato Mild Mottle Virus from Tree Tomato and their Distribution in Kenya
dc.type	Article