Comparative evaluation of anticoagulatory activity of ethylenediamine tetra-acetic acid (EDTA) and heparin for haematological analysis

Abstract

Sample collection for haematological analysis requires use of anticoagulant, the two commonly used in trypanosomiasis research being ethylenediamine tetra-acetic acid and heparin. Since these are known to have different modes of action (Lewis, 200 I), it is important to ascertain whether they can be used alternatively. Further, Swiss White mouse has been used in research involving disease pathogenesis and trials of new drugs (Kibugu et al., 2009, Thuita et al., 2008). Since handling methods are important for accurate haematological results, appropriate blood sampling and processing techniques need to be employed. The small body size of the mouse is probably a limitation to blood sample collection. The aim of the present study was to evaluate the anticoagulatory efficacy of ethylenediamine tetra-acetic acid and heparin for electronic cell counting, and develop a suitable blood handling procedure for laboratory mice.