Ahmed, J. S.Winter, P.Olaho-Mukani, W.Dorflinger,W.Horchner, F.2015-08-182015-08-181990Olaho-Mukani, W., Winter, P., Dörflinger, W., Hörchner, F., & Ahmed, J. S. (1990). Application of luminol-dependent chemiluminescence to assay opsonizing antibodies to procyclic forms of Trypanosoma congolense in the sera of dogs experimentally infected with heterologous stocks. Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), 41(2), 213–216. https://pubmed.ncbi.nlm.nih.gov/2382102/0031-1820https://kalroerepository.kalro.org/handle/0/10142Luminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as I: 400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.enhttp://creativecommons.org/licenses/by/3.0/Journal ContributionChemiluminescence detectionantibodiesTrypanosoma congolenseAgglutination tests