Karanja, W. M.Mdachi, R. E.Murilla, G. A.Kamidi, C. M.Muchiri, M. W.2015-08-172015-08-172010Karanja, W. M., Mdachi, R. E., Murilla, G. A., TKamidi, C. M., Muchiri, M. W. (2010). Enzyme- Linked Immunosorbent Assay (ELISA) for Determination of Homidium Residues in Meat Animal Health And Production, 1, https://www.researchgate.net/publication/2611341580049-4747https://kalroerepository.kalro.org/handle/0/9913Homidium bromide has been commonly used since 1950s to treat trypanosomosis in cattle. Due to its widespread use, there is a risk of residues in foods of animal origin. Due to the fact that homidium is a suspected carcinogenic and teratogenic agent, it has no allowed minimum residue level (MRL) and hence, no amount of the drug is allowed in food. It is therefore necessary to have a suitable analytical technique that is sensitive, simple, cost effective and rapid for detection of homidium residues. A direct competitive enzyme-linked immunosorbent assay (ELISA) was performed to determine homidium presence in skeletal muscle tissue extract. The limit of detection and quantification were 1ng/ml and 2.6ng/ml respectively. The coefficients of intra-assay and inter-assay variation were less than 10%. Recoveries above 70% were obtained using simple and rapid extraction method utilizing cheap buffers. Assay accuracies for tissue extract spiked at 1ng/ml and 2.5ng/ml were above 90%. The homidium ELISA has a high throughput and therefore suitable for screening large amounts of samples for homidium residues. This would be a major step in safeguarding consumers against the harmful effects of homidiumenhttp://creativecommons.org/licenses/by/3.0/Journal ContributionEnzymesEnzyme linked immunosorbent assayResiduesTrypanosomiasis