Olaho M. W.Winter, P.Dorflinger, W.Ahmed, J. S.2015-08-132015-08-131972Olaho-Mukani, W., Winter, P., Dörflinger, W., Hörchner, F., & Ahmed, J. S. (1972). Application of Luminol-Dependent Chemiluminescence to Assay Opsonizing Antibodies to Procyclic Forms of Trypanosoma Congolense in the Sera of Dogs Experimentally Infected with Heterologous Stocks. Tropical Medicine and Parasitology: Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), 41(2), 213-216.https://kalroerepository.kalro.org/handle/0/9496Luminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as I: 400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.enhttp://creativecommons.org/licenses/by/3.0/Journal ContributionGranulocytesAntibodiesTechniquesTrypanosoma