Masiga, D.K.McNamara, J.J.Laveissiere, C.Truc, P.Gibson, C.W.2015-08-182015-08-181996Masiga, D. K., McNamara, J. J., Laveissière, C., Truc, P., & Gibson, W. C. (1996). A high prevalence of mixed trypanosome infections in tsetse flies in Sinfra, Cote d'Ivoire, detected by DNA amplification. Parasitology, 112(1), 75-80. https://doi.org/10.1017/s00311820000650940049-4747https://kalroerepository.kalro.org/handle/0/10102The prevalence of various species and subgroups of trypanosomes in the Sinfra area of Cote d' I voir was determined using the polymerase chain reaction (PCR). Using this technique to amplify specific satellite DNA, targets, it was possible. To identify developmental-stage trypanosomes in the midguts and the proboscides of tsetse without expansion .of parasite populations. The predominant tsetse species in the area was Glossina palpalis, which G pallicera and G. nigrofusca were also present. Microscopical examination of 811 non- teneral flies revealed an infection rate of 14% in midguts and or proboscides. Three subgroups of Trypanosoma congolense (Savannah, Forest & Kilifi), T. simiae, T. gadfreyi, West African T vivax and T brucei ssp. were identified using PCR. T. congolense Forest was the most abundant of the Nannomonas trypanosomes approximately 40 % of all infections were mixed, and there was a significantly higher prevalence of apparently mature T brucei ssp. trypanosomes than has previously been reported. The present study demonstrates the PCR facilitates the easy Identification of mature trypanosome infections in tsetse, providing a reliable estimation of trypanosomiasis challenge.enhttp://creativecommons.org/licenses/by/3.0/Journal ContributionInfectionTsetse fliesDNATrypanosomiasisPolymerase chain reactionhttps://doi.org/10.1017/s0031182000065094