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Bulletin of Animal Health and Production in Africa Bulletin De La Sante Et De La Production Animales En Afrique
(African Union, 2009) African Union
A recombinant hsp 70/Bip antigen for detection of bovine trypanosomosis was evaluated as a means of diagnosis using bovine field serum samples from Kachia grazing reseNe, Nigeria. Circulating anti trypanosomal antibodies was detected in 359 of 545 sera using inhibition ELISA in cattle with apparent serological prevalence of 65.9% as against 6.1 % prevalence overall by buffy coat method. Of 33 serum samples from cattle with parasitological confirmed diagnosis, 28 had circulating anti-trypanosomal antibodies giving an apparent sensitivity of 84.8%. The apparent specificity was 97.3%. Further specificity of the assay was based on the results of analysis performed on sera samples from cattle infected with Theileria parva, Anaplasma marginale and Babesia bigemina, which tested all negative in the assay. In addition, returned herds and resident herds had significant anti-trypanosoma I antibodies and the test was able to detect antibodies of three trypanosomes species T. vivax, T. congolense and T. b. brucei. Based on the findings, the test offers promise as a useful tool in sero- diagnosis of bovine trypanosomosis. However, improvement in test sensitivity and further evaluation of the assay are suggested.