Browsing by Author "Gumm, I. D."
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Item Growth of Rinderpest and Bovine Virus Diarrhea Viruses in Theileria Parva Infected Iympho blastoid Cell Lines(1988) Rossiter, P.B.; Wafula, J.S.; Gumm, I. D.CO-Cultivation of lymphoid cells from infected cattle on feeder monolayers of bovine spleen or thymus is a routine technique for establishing continuous lines of lymphoblastoid cells transformed and infected by the protozoan Theileria parva (Malmquist and Brown 1977, Kurtti and others 1981). Recently several viruses, including bovme herpesvlfus-3 (also classified as bovid herpesvirus-4) and rinderpest vaccine virus have been isolated from such cultures following development of cytopathic effects in the monolayers (P. B. ROSSISTER and others, m preparation). It would have been interesting to know whether these viruses originated from the monolayers or the transformed Iympho blasts or other cells in the culture but it was not possible to determine this. A small study was subsequently made to investigate whether two lympho tropic viruses, rinderpest virus and Bovine virus diarrhoea virus (BVDV) could infect and grow in T parvaI~ infected Iympho blastoid cell lines which, once established, grow continuously in suspension without a requirement for feeder layers.Item In vitro development of metacyclic Trypanosoma simiae derived from bloodstream trypomastigotes(1989) Zweygarth, E.; Gumm, I. D.; Gray M. A.; Cheruiyot, J. K.; Webster , P.; Kaminsky, R.; Kenya Trypanosomiasis Research InstituteA stock of Trypanosoma simiae was transformed into procyclic forms at 26°C in a semi-defined maintenance medium. After transformation, the trypanosomes were maintained in a modified Eagle's MEM medium. On day 35 of cultivation, epimastigotes attached to the bottom of the culture flask. From day 44 onwards, metacyclic-like trypanosomes were observed. Subcutaneous injections into pIgs of trypanosome suspensions obtained from cultures on day JO were not Infective, whereas culture-derived metacyclics (days 44, 63 and 69) were highly pathogenic.Item Use of Vero Cells for the Isolation And Propagation of Malignant Catarrhal Fever Virus(1987) Rossiter, P. B.; Gumm, I. D.; Wamwayi, H. M.Vero cells were compared with primary bovine thyroid (BTh) cultures for the isolation of malignant catarrhal fever virus from infected blood and tissues. Comparative titrations showed Vero cells detected only two-fold less infectivity in rabbit spleen suspensions than BTh cells. Twenty three of 32 bovine buffy coat cell preparations which were positive on BTh cells were all also positive on Vero cells. The cytopathic effects (CPE) of virus isolates in Vero cells consisted of syncytia and refractile cytomegalic cells which were as easy to recognise and developed as rapidly as CPE in BTh cells. Two laboratory strains of malignant catarrhal fever virus were readily adapted to and maintained by passage in Vero cells.
