Browsing by Author "Palmer, R.F."
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Item The Antigenicity of Mycoplasma mycoides III-Isolation of Precipitating Antigens from Urine.(1965) Gourlay, R.N.; Palmer, R.F.; East African Veterinary Research Organisation, Muguga, P.O. Box 32, Kikuyu, KenyaPrecipitation of urine with increasing volumes of iso-propyl alcohol, removing the precipitate at each step and deproteinization with chloroform and butanol, produced a number of fractions which gave specific precipitin hands in the agar gel precipitin test when diffused against hyperimmune sheep sera, and which were Molisch positive and biuret negative. The number of precipitin bands produced with the different fractious depended on the amount of alcohol used for the precipitation. In a series of experiments in which the precipitate produced by each volume of alcohol was removed before the next volume of alcohol was added, 1 and 2 volumes of alcohol produced fractions which gave 6 precipitin bands, while 3 volumes produced a precipitate which gave 4 hands or occasionally 5. Precipitates from 4 and 5volumes produced 4 bands, whereas 6 volumes gave only 2 bands. P.1/2/3, the result of precipitation with 3 volumes of alcohol added in one aliquot, gave 6 precipitin bands, whereas P.4/5/6, the result of precipitation with a further 3 volumes of alcohol followed by alternate precipitation with 3 and 6 volumes, gave 2 strong and 2 weak precipitin bands.Item Further Studies on the Allergic Reaction In Contagious Bovine Pleuropneumonia(1965) Gourlay, R.N.; Palmer, R.F.; East African Veterinary Research Organization, Muguga, KenyaAn earlier paper (Gourlay, 1964) recorded the preparation of an allergic antigen and its use, both experimentally and in the field, for the diagnosis of Contagious Bovine Pleuropneumonia (CBPP). This antigen contained a certain amount of insoluble material that possessed activity. The present paper describes further work done in an effort to produce a more soluble antigen, the use of a more virulent strain of Mycoplasma mycoides for antigen production and its use experimentally and in the field.Item Lyophilisation of Mycoplasma Culture Vaccine(1964) Palmer, R.F.; Gourlay, R.N.; East African Veterinary Research Organization, MugugaLiquid culture vaccines against contagious bovine pleuropneumonia are used in various parts of Mrica and Australia. Lyophilisation provides an obvious method of overcoming the poor keeping properties of these vaccines and furthermore, by lyophilisation a properly tested and standardised product can be issued and stocks accumulated to meet sudden heavy demands. A series of experiments were undertaken, using two culture vaccine strains of M. mycoides, to compare various suspending media, two methods of lyophilisation and different reconstituting fluids. A reconstitution curve using the most suitable of these methods was also prepared.
