Browsing by Author "Rowe, L.W."
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Item Cell Fusion Using Sendai Virus to Effect Interspecies Transfer of Cell Associated Parasite(1974) Irvin, A.D.; Brown, C.G.D.; Kanhai,G. K.; Stagg, D.A.; Rowe, L.W.; Institute for Research on Animal Diseases, Compton, United Kingdom; FAO ; Central Veterinary Laboratory, United Kingdom; Central Veterinary LaboratoryBaby hamster kidney (BHK) cells were fused with Theileria parva-infecled bovine lymphoid cells, using u.v. light-inactivated Sendai virus. The resultant hamster/bovine heterokaryons were shown to be infected with T. parva. In some cases parasites were detected in cells which apparently contained only BHK nuclei.Item Hybrid Cells, Infected with Theileria Parva Formed by Fusion Hamster and Mouse Cells with Parasitised Bovine Lymphoid Cells.(1975) Brown, C.G.D; Stagg, D.A.; Irvin, A.D.; Kanhai, G. K.; Rowe, L.W.; East African Veterinary Research OrganisationBovine lymphoid cells from a culture line parasitised with Theileria were fused to mouse heart and baby hamster kidney cells grown as monolayers using inactivated Sendai virus the resultant heterokaryons contained macroschizonts of T.Parva .Item Hybrid Cells, Infected with Theileria Parva, Formed by Fusion of Hamster and Mouse Cells with Parasitised Bovine Lymphoid Cells(1975) Irvin, A.D.; Brown, C.O.D.; Stagg, D.A.; Kanhai, O.K.; Rowe, L.W.; UNDP/FAO Tick-borne Diseases Project, East African Veterinary Research Organisation, Muguga, KenyaBovine lymphoid cells from a culture line parasitised with Theileria parva were fused to mouse heart (MH) and baby hamster kidney (BHK) cells, grown as monolayers, using inactivated Sendai virus. The resultant heterokaryons contained macro schizonts of T parva. Macro schizonts also occurred in cells that apparently contained only BHK or MH nuclei. Parasites underwent varying degrees of development; in some cases, micro schizonts resulted, and in other cases massive atypical parasite masses were formed. Fusion occurred more readily between hamster and bovine cells than between mouse and bovine cells.
