The Indirect Fluorescent Antibody Test for Experimental East Coast Fever (Theileria parva Infection of Cattle). Evaluation of a Cell Culture Schizont Antigen

Abstract

A schizont antige/l for the indirect fluorescent antibody test was prepared from all in vitro culture suspension of lymphoid cells infected with Theileria parva macroschizonts. The fluorescence of the intracellular schizonts was bright and specific with T. parva positive control serum, but absellt with either conjugate alone or negative cOlltrol serum. Background stailling of the cells was minimal. No serological cross-reaction was detected with sera from cattle infected with T. mutans piroplasms.