Simple Techniques for Increasing the Batch Size of Rinderpest Cell Culture Vaccine

Abstract

Vaccine production could be increased by culturing monolayers around the complete circumference of the flask, by seeding with three times the usual amount of cells and virus followed by continuous rolling, and by pooling two harvests. Increasing the volume of medium threefold before harvest caused a two-fold decrease in titre. Only very high titre harvests should be stored at -70°C before freeze drying, otherwise short-term storage at 4°C is recommended.