Infection and Transformation of Bovine Lymphoid Cells In Vitro by Infective Particles of Theileria Parva

Abstract

Techniques recently developed in this laboratory can be used to infect cattle reproducibly with East Coast fever with infective particles of Theileria parva collected from the tick vector, Rhipicephalus appendiculatus. Infective particles can be collected either as in vitro tick feed material by inducing prefed, infected ticks to salivate into capillary tubes containing foetal calf serum1, or as ground tick supernatant obtained by grinding the ticks in Eagle's minimum essential medium with Earle's salts (MEM) supplemented with bovine plasma albumin (Fraction V from bovine plasma) (BPA) and collecting the supernatant2.