Lyophilisation of Mycoplasma Culture Vaccine

Abstract

Liquid culture vaccines against contagious bovine pleuropneumonia are used in various parts of Mrica and Australia. Lyophilisation provides an obvious method of overcoming the poor keeping properties of these vaccines and furthermore, by lyophilisation a properly tested and standardised product can be issued and stocks accumulated to meet sudden heavy demands. A series of experiments were undertaken, using two culture vaccine strains of M. mycoides, to compare various suspending media, two methods of lyophilisation and different reconstituting fluids. A reconstitution curve using the most suitable of these methods was also prepared.