Animal Health
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Browsing Animal Health by Author "Ahmed, J. S."
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Item Application of Luminol-Dependent Chemiluminescence Opsonizing Antibodies to Procyclic Forms of Trypanosoma Congolense in the Sera of Dogs Experimentally Infected with Heterologous Stocks(veterinary Record, 1972) Olaho M. W.; Winter, P.; Dorflinger, W.; Ahmed, J. S.; Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität BerlinLuminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as I: 400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.Item Application of luminol-dependent chemiluminescence to assay opsonizing antibodies to procyclic forms of Trypanosoma congolense in the sera of dogs experimentally infected with heterologous stocks(1990) Ahmed, J. S.; Winter, P.; Olaho-Mukani, W.; Dorflinger,W.; Horchner, F.; Kenya Trypanosomiasis Research Institute; Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ),Luminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as I: 400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.
