Animal Health
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Browsing Animal Health by Author "Allsopp, B. A."
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Item Monosaccharide composition of the surface glycoprotein antigens of Trypanosoma brucei(1974) Allsopp, B. A.; Njogu, A. R.; East African Trypanosomiasis Research Organization, Tororo, UgandaEvidence has been adduced that the surface antigens of Trypanosoma brucei subgroup are a group of glycoproteins having D-galactose, D-mannose and D-glucosamine as monosaccharide components. There was considerable variation in the number of antigen components, and their relative amounts, as revealed by isoelectric focusing on polyacrylamide gel. The relevance of these variations to the adaptation theory of antigenic variation in trypanosomes is discussed.Item Nature and Location of Trypanosoma bruce Subgroup Exoantigen and Its Relationship to 4S Antigen(1971) Allsopp, B. A.; Njogu, A. R.; Humphreys, K.C.; East African Trypanosomiasis Research Organisation, Tororo, UgandaIt has been suggested that exoantigen of Trypanosoma brucei subgroup is not continuously secreted by trypanosomes in the bloodstream of hosts. The antigen was not detected in plasma collected quickly, but appeared in increasing quantities when blood was removed from the host and allowed to stand. Analysis of exoantigen by isoelectric focusing in polyacrylamide gel has shown it to be a heterogeneous antigen of at least three components with a common antigenic determinant. Each of the components is a protein carbohydrate complex, having an isoelectric point (pI) in the pH range 6.0–5.5. Evidence was presented suggesting that this antigen is identical to 4S antigen, AND has its main location as a major component of the surface coat of bloodstream forms of T. brucei subgroup. The name “surface antigen” has been proposed, AND a method for rapidly preparing it in an apparently fairly pure form from trypanosomal homogenates has been detailed.
