Animal Health
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Browsing Animal Health by Subject "Agglutination tests"
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Item The Allergic Reaction in Contagious Bovine Pleuropneumonia(1964) Gourlay, R.N.; East African Veterinary Research Organization, Muguga KenyaSerological diagnosis of Contagious Bovine Pleuropneumonia (CBPP) has been primarily by means of the complement fixation test (CFT), but also to a lesser extent by the slide agglutination serum test (SAST), the slide agglutination blood test (SABT) and the agar gel double diffusion precipitin test (AGT). All these tests, except possibly the slide agglutination tests, require some laboratory facilities and the case of the CFT quite elaborate ones. In addition, all these tests, except possibly the SABT, necessitate the numbering of each animal for subsequent identification. These factors form a serious obstacle to the diagnosis of CBPP in some countries of Africa due to the vast distances between the laboratory and outbreaks of the disease and the lack of communications and transport. The development, therefore, of a simple diagnostic test, along the lines of the tuberculin test, has had a high priority in research programmes on CBPP in East Africa.Item Application of luminol-dependent chemiluminescence to assay opsonizing antibodies to procyclic forms of Trypanosoma congolense in the sera of dogs experimentally infected with heterologous stocks(1990) Ahmed, J. S.; Winter, P.; Olaho-Mukani, W.; Dorflinger,W.; Horchner, F.; Kenya Trypanosomiasis Research Institute; Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ),Luminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as I: 400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.Item Surveillance of African Trypanosomiasis(1972) East Africa Trypanosomiasis Research OrganizationAfrican trypanosomiasis is exceptional, particularly amongst parasitic diseases, in that it is almost invariably fatal if untreated. The devastating epidemics of the first half of this century, caught the attention of both medical authorities and the public and gave sleeping sickness and tsetse their dreaded reputation. However, also in all the countries of central and western Africa through which the epidemic wave passed, a sleeping sickness control organization was set up which later, when reasonable control had been attained, expanded to become a service dealing with the major epidemic diseases encountered in rural conditions.
